Recently, Ph.D. student Yan Gong from BEBC has published her first author paper in International Journal of Nanomedicine entitled "Improved Lateral Flow Immunoassays for Highly-Sensitive Detection of Brain Natriuretic Peptide at the Point of Care ".

Heart failure (HF) has become a major cause of morbidity and mortality with a significant global economic burden. Although well-established clinical tests could provide early diagnosis, access to these tests is limited in developing countries where a relatively higher incidence of HF is present. This has prompted an urgent need for developing a cost-effective, rapid and robust diagnostic tool for point-of-care (POC) detection of HF. Lateral flow immunoassay (LFIA) has found widespread applications in POC diagnostics. However, the low sensitivity of LFIA limits its ability to detect important HF biomarkers (e.g., BNP) that are normally present in low concentration in blood. To address this issue, we developed an improved LFIA by optimizing the gold nanoparticle-antibody conjugate conditions, the diameter of GNP and the concentration of antibody at the test line, and modifying the structure of test strip. Through these improvements, the proposed test strip enables the detection of BNP down to 0.1 ng/mL within 10-15 minutes, presenting ~15-fold sensitivity enhancement over conventional lateral flow assay. We also successfully applied our LFIA in the analysis of BNP in human serum samples, highlighting its potential use for clinical assessment of HF. The developed LFIA for BNP could rapidly rule out HF with the naked eye, offering tremendous potential for POC and personalized medicine.

The work is under the guidance of Professor Feng Xu and Dr. Ting Wen. The co-authors of the paper include Jie Hu, Jane Ru Choi, Minli You, Yamin Zheng and Bo Xu.